Michaelis Menten parameters estimation of p-nitrophenyl phosphate

I need an abstract, result & discussion and conclusion for my final report( named final report)

subject is : Michaelis Menten parameters estimation for reaction of p-nitrophenyl phosphate (pNPP) as catalyzed by the enzyme alkaline phosphatase at two different enzyme concentrations

you might use the example report to help you (named example but be careful for the objective it may vary, also don’t copy from it).

you will find the data required in excel files. Also, make sure to fix all the comment in the final report.

please follow the objective and do the required tests and tasks.

following is how to write each section: (rubric)


Key features of the report in about 250 words (quarter page, single spaced.)

  • Reason for the study (often left out, especially when “it was assigned” is the reason)
  • Experimental objective
  • Equipment and methods used – think unique and novel, think scale
  • Types and ranges of data collected – think experimental design
  • Principal results
  • Major conclusions
  • ——————————————————————————————————–

    Results and Discussion (can be two sections) .

    • Presentation of the data

    • Presentation of all calculated results

    • Discussion of the meaning of the results



    A conclusion paragraph contains a description of the purpose of the experiment, a discussion of your major findings, an explanation of your findings, and recommendations for further study. Address the following points in paragraph form (don’t just number off and answer each question)

    1. Restate the overall purpose of the experiment.

    a. One format: The purpose of the experiment was to investigate the effect of (THIS) on (THAT)

    b. Example: The purpose of the experiment was to investigate the effect of stress on the growth of bean plants by comparing the growth of bean plants subjected to stress for 15 days with a control (non-stressed plants.)

    2. What were the major findings? (Summarize your data and graph results)

    a. Example: No significant difference existed between the height of stressed plants and non-stressed plants. As the graph shows above, the average height of all the stressed plants was 10.2 cm and the average height of all the non-stressed plants was 10.4 cm.

    3. Was the hypothesis supported by the data?

    a. One format: The hypothesis that (insert your hypothesis) was (supported, partially supported, or not supported.) Please do not ever use the word “prove” – we do NOT prove hypotheses true in science.

    b. Example: The hypothesis that stressed plants would have a dramatically lower mean height was not supported.

    4. How could this experiment be improved?

    a. Example: This experiment relied on an artificial source of stress – just digging out the plants at one time and replanting them. Perhaps this experiment could be improved by simulating real-life stressors, including drought and lack of nutrients in soil.

    b. NOT acceptable: This experiment would have been better if we had done it correctly – we did sloppy work and made careless measurements.

    5. What could be studied next after this experiment? What new experiment could continue study of this topic?

    a. Example: Additional investigations using various sources of stress at more frequent intervals would be a good additional experiment. Also, other crops could be subjected to the same experiment, such as corn and squash. Perhaps scientists could find a chemical that the plants release during stress.

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